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Related Concept Videos

Protein Organization01:24

Protein Organization

Proteins are polymers of amino acid residues. They are versatile and responsible for different cellular functions, including DNA replication, molecular transport, catalysis, and structural support. Proteins have a hierarchical structure comprising at least three levels of organization: primary, secondary, and tertiary structure. Some large proteins have a quaternary structure where individual protein subunits are linked together.
The primary structure of a protein is its amino acid sequence.
Protein Organization01:13

Protein Organization

Overview
Protein Organization01:24

Protein Organization

Proteins are polymers of amino acid residues. They are versatile and responsible for different cellular functions, including DNA replication, molecular transport, catalysis, and structural support. Proteins have a hierarchical structure comprising at least three levels of organization: primary, secondary, and tertiary structure. Some large proteins have a quaternary structure where individual protein subunits are linked together.
The primary structure of a protein is its amino acid sequence.
Protein Organization01:13

Protein Organization

Overview
Protein and Protein Structure02:15

Protein and Protein Structure

Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme can...
Protein Folding01:22

Protein Folding

Overview

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Application of I TASSER, trRosetta, UCSF Chimera, HADDOCK server, and HEX loria for De Novo and In Silico Design of Proteins
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Application of I TASSER, trRosetta, UCSF Chimera, HADDOCK server, and HEX loria for De Novo and In Silico Design of Proteins

Published on: July 8, 2025

Studying protein structure and function using semisynthesis.

Tom W Muir1

  • 1Laboratory of Synthetic Protein Chemistry, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA. muirt@mail.rockefeller.edu

Biopolymers
|October 17, 2008
PubMed
Summary
This summary is machine-generated.

Protein semisynthesis using Expressed Protein Ligation (EPL) enables the creation of modified proteins. This technique has been instrumental in solving complex biochemical problems and studying protein modifications.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Chemical Biology

Background:

  • Protein engineering and modification are crucial for understanding biological functions.
  • Traditional methods for protein synthesis have limitations in creating complex or modified proteins.

Purpose of the Study:

  • To review the development and application of Expressed Protein Ligation (EPL) for protein semisynthesis.
  • To highlight the utility of EPL in solving biochemical problems and studying protein post-translational modifications.

Main Methods:

  • Expressed Protein Ligation (EPL) technique.
  • Assembly of modified proteins from recombinant and synthetic peptide building blocks.
  • Application of EPL to study protein post-translational modifications.

Main Results:

  • EPL allows the construction of complex, modified proteins.
  • The technique has been widely adopted since its introduction in 1998.
  • EPL facilitates the investigation of protein post-translational modifications.

Conclusions:

  • Expressed Protein Ligation is a powerful tool for protein semisynthesis.
  • EPL has broad applications in biochemistry and molecular biology.
  • The technique is particularly valuable for studying protein post-translational modifications.