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Updated: Jun 28, 2026

Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)

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A PC-controlled module system for automatic sample preparation and analysis.

O Einarsson¹, L Hansén

¹Analys Modul AB S191 62 Sollentuna Sweden.

The Journal of Automatic Chemistry

|January 1, 1995

Summary

This summary is machine-generated.

A new automated liquid chromatography system offers a cost-effective and reproducible method for analyzing primary amines using pre-column derivatization and fluorescent detection.

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Area of Science:

Analytical Chemistry
Chromatography

Background:

Automated analytical systems enhance efficiency and reproducibility in chemical analysis.
Liquid chromatography (LC) is a key technique for separating and quantifying chemical compounds.

Purpose of the Study:

To construct and evaluate a simple, modular automated analytical system for liquid chromatography.
To apply the automated system for the analysis of primary amines.

Main Methods:

Construction of an automated system with modules including auto sampler, auto dispenser, selector valve, heater/cooler, mixing chamber, and injector valve.
PC-controlled process with a changeable run protocol.
Pre-column derivatization of primary amines followed by isocratic reversed-phase HPLC with fluorescent detection.

Main Results:

The automated system demonstrated good reproducibility and analytical precision.
Comparison showed comparable or improved results from automated versus manual derivatization and injection.
The system was found to be easy to handle and cost-effective.

Conclusions:

The developed automated liquid chromatography system is a viable, efficient, and cost-effective tool for primary amine analysis.
The system offers good reproducibility, making it suitable for routine analytical applications.