Related Experiment Video
Updated: Jun 28, 2026
12:11
Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
Published on: February 27, 2020
On-line analyte preconcentration with atomic spectrometric detection.
L Ebdon1, A S Fisher, S J Hill
1Plymouth Analytical Chemistry Research Unit Department of Environmental Sciences Polytechnic South West Drake Circus, Devon Plymouth PL4 8AA UK.
The Journal of Automatic Chemistry
|January 1, 1991
Summary
Mini- and micro-columns with exchange media are increasingly used for analyte pre-concentration and matrix removal before atomic spectrometric detection. This review covers recent applications of chelating, ion exchange, and other resins and gels for these purposes.
Area of Science:
- Analytical Chemistry
- Separation Science
Background:
- Atomic spectrometric detection often requires analyte pre-concentration.
- Matrix interferences can significantly impact detection accuracy.
- Mini- and micro-column separation techniques offer efficient solutions.
Purpose of the Study:
- To review recent advancements in using mini- and micro-columns for analyte pre-concentration.
- To explore matrix removal strategies using various exchange media.
- To highlight applications in atomic spectrometry.
Main Methods:
- Review of recent scientific literature on column-based separation techniques.
- Focus on chelating resins, ion exchange resins, and other sorbent materials.
- Application in pre-concentration and matrix removal prior to analysis.
Main Results:
- Demonstrated effectiveness of mini- and micro-columns for analyte enrichment.
- Successful matrix interference mitigation using diverse resin types.
- Broad applicability across various atomic spectrometric methods.
Conclusions:
- Mini- and micro-columns are valuable tools for enhancing atomic spectrometry.
- Diverse resins and gels provide versatile options for sample preparation.
- These techniques are crucial for accurate and sensitive elemental analysis.
Related Concept Videos
01:21Atomic Absorption Spectroscopy: Lab
For AAS measurements, samples must be introduced as clear solutions, often requiring extensive preliminary treatment to dissolve materials like soils, animal tissues, and minerals. Common methods for sample preparation include treatment with hot mineral acids, wet ashing, combustion in closed containers, high-temperature ashing, or fusion with reagents.
Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing nebulizer...
Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing nebulizer...
01:29Atomic Emission Spectroscopy: Lab
AES is a powerful analytical technique, especially effective when used with plasma sources, producing abundant spectra in characteristic emission lines. The Inductively Coupled Plasma (ICP), in particular, yields superior quantitative analytical data due to its high stability, low noise, low background, and minimal interferences under optimal experimental conditions. However, newer air-operated microwave sources are emerging as promising alternatives that could be more cost-effective than...
01:25Atomic Absorption Spectroscopy: Interference
Interference leads to systematic error in atomic absorption (AA) measurements by enhancing or diminishing the analytical signal or the background. These interferences can be grouped into three main categories: spectral interference, chemical interference, and physical interference.
Spectral interference occurs when signals from other elements or molecules overlap with the analyte signal, falsely elevating or masking the analyte's absorbance. This interference can be corrected using Zeeman,...
Spectral interference occurs when signals from other elements or molecules overlap with the analyte signal, falsely elevating or masking the analyte's absorbance. This interference can be corrected using Zeeman,...
01:22Atomic Emission Spectroscopy: Instrumentation
The instrumentation of atomic emission spectrometry (AES) involves various components, including atomization devices that convert samples into gas-phase atoms and ions. There are two main types of atomization devices: continuous and discrete atomizers. Continuous atomizers, like plasmas and flames, introduce samples in a constant stream, while discrete atomizers inject individual samples using syringes or autosamplers. The most common discrete atomizer is the electrothermal atomizer.
01:20Atomic Emission Spectroscopy: Overview
Atomic emission spectroscopy (AES) is an analytical technique used to determine the elemental composition of a sample by analyzing the light emitted from excited atoms. In AES, atoms in a sample are excited to higher energy levels by thermal energy from high-temperature sources, such as plasma, arcs, or sparks. When these excited atoms return to lower energy states, they emit light at specific wavelengths characteristic of each element. The resulting atomic emission spectrum, which consists of...
01:27Atomic Absorption Spectroscopy: Overview
Atomic absorption spectroscopy (AAS) is a technique used to analyze elements by measuring electromagnetic radiation (EMR) absorbed by atoms, which causes them to transition to a higher-energy orbit. The most crucial step in AAS is atomization, where the analyte is converted into gas-phase atoms, typically through a flame or furnace. Some of these atoms become thermally excited in the flame, while most remain in the ground state.
When irradiated by EMR of a particular wavelength, these...
When irradiated by EMR of a particular wavelength, these...