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Performance characteristics of a light-scattering immunoassay for thyroxine on a discretionary analyser

R Jaggon1, C P Price

  • 1Department of Clinical Biochemistry Addenbrooke's Hospital Hills Road Cambridge CB2 2QR UK.

The Journal of Automatic Chemistry
|January 1, 1987
PubMed
Abstract

No abstract available in PubMed .

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Enzyme-Linked Immunosorbent Assay01:33

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In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.