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Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy
09:21

Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy

Published on: July 20, 2019

Light microscopic methods to visualize mitochondria on tissue sections.

Kurenai Tanji1, Eduardo Bonilla

  • 1Department of Pathology, Columbia University, New York, NY 10032, USA.

Methods (San Diego, Calif.)
|October 22, 2008
PubMed
Summary
This summary is machine-generated.

Mitochondria, vital for cell energy, are implicated in aging and diseases. This study reviews reliable methods for visualizing mitochondria in tissue, aiding disease research.

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Area of Science:

  • Cell Biology
  • Biochemistry
  • Pathology

Background:

  • Mitochondria are double-membrane organelles crucial for cellular ATP synthesis.
  • Mitochondrial dysfunction is increasingly recognized as a cause of neurological, cardiac, and age-related diseases, including cancer.
  • Advances in molecular genetics, biochemistry, and cell biology have enhanced our understanding of mitochondrial roles.

Purpose of the Study:

  • To review and present reliable histochemical and immunohistochemical methods for visualizing mitochondria.
  • To provide updated protocols for analyzing mitochondria in normal and diseased tissue specimens.
  • To support research into mitochondrial disorders, aging, and neurodegenerative diseases.

Main Methods:

  • Two histochemical staining techniques.
  • Three immunohistochemical staining techniques.
  • Detailed protocol descriptions and illustrations for each method.

Main Results:

  • Identification of five reliable methods (two histochemical, three immunohistochemical) for mitochondrial visualization.
  • Demonstration of these methods' efficacy on normal and disease tissue sections.
  • Provision of updated, practical protocols for researchers.

Conclusions:

  • Histochemical and immunohistochemical methods are essential tools for studying mitochondrial structure and function in disease.
  • The reviewed protocols offer reliable approaches for visualizing mitochondria in pathological specimens.
  • Accurate visualization of mitochondria aids in understanding their role in various diseases and aging.