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DNA sequencing by denaturation: principle and thermodynamic simulations

Ying-Ja Chen1, Xiaohua Huang

  • 1Department of Bioengineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0412, USA.

Analytical Biochemistry
|October 22, 2008
PubMed
Summary

A novel DNA sequencing by denaturation (SBD) method decodes base sequences by analyzing sequential DNA fragment denaturation profiles. This approach enables rapid, cost-effective, large-scale genome resequencing with millions of parallel reactions.

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