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Updated: Jun 28, 2026
07:57
Fish Sperm Assessment Using Software and Cooling Devices
Published on: July 28, 2018
FISH on sperm: spot-counting to stop counting? Not yet.
Òscar Molina1, Zaida Sarrate1, Francesca Vidal1
1Unitat de Biologia Cel·lular (Facultat de Biociències), Universitat Autònoma de Barcelona, Bellaterra (Cerdanyola del Vallès), Spain.
Fertility and Sterility
|October 22, 2008
Summary
The Cytovision Spot AX workstation shows promise for sperm fluorescence in situ hybridization (FISH) analysis, demonstrating high reliability in detecting anomalies. However, further improvements are needed before widespread clinical adoption.
Area of Science:
- Reproductive biology
- Genetics
- Medical technology
Background:
- Sperm fluorescence in situ hybridization (FISH) is crucial for assessing male infertility.
- Manual FISH analysis is time-consuming and labor-intensive.
- Automated systems offer a potential solution to improve efficiency.
Purpose of the Study:
- To evaluate the reliability and applicability of the Cytovision Spot AX workstation for sperm FISH analysis.
- To compare automated spot-counting with traditional manual counting methods.
Main Methods:
- Semen samples from 24 men with varying seminal parameters were analyzed.
- Dual-color and triple-color FISH were performed on sperm samples.
- Manual FISH counts were compared with automated counts from the Spot AX system.
Main Results:
- The automated system achieved 96.67% agreement with manual counts across 120 comparisons.
- Statistical differences were observed in only 3.33% of cases.
- Automated analysis time was generally longer than manual analysis, depending on sample preparation.
Conclusions:
- The Cytovision Spot AX system demonstrates significant potential for reliable sperm FISH analysis.
- Limitations related to analysis time and specific preparation characteristics require further investigation.
- The system needs further refinement before it can be fully implemented in clinical services.
