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Protocols for nuclei isolation and nuclear protein extraction from the resurrection plant Xerophyta viscosa for
Kamal Omer Abdalla1, Jennifer Ann Thomson, Muhammad Suhail Rafudeen
1Department of Molecular and Cell Biology, University of Cape Town, Private Bag, Rondebosch 7701, South Africa.
Analytical Biochemistry
|October 22, 2008
Abstract:
The plant nucleus is an important subcellular organelle but the isolation of pure and enriched nuclei from plants and subsequent extraction of nuclear proteins for proteomic studies is challenging. Here, we present protocols for nuclei isolation and nuclear protein extraction from the resurrection plant, Xerophyta viscosa, and show optimization and modification of the most critical steps.
