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Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing

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One-shot lactate chemiluminescent biosensor.

J Ballesta-Claver¹, M C Valencia-Mirón, L F Capitán-Vallvey

¹Solid Phase Spectrometry Research Group, Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Campus Fuentenueva, Granada E-18071, Spain.

Analytica Chimica Acta

|October 23, 2008

Summary

A novel one-shot biosensor utilizes chemiluminescence for rapid lactate detection. This inexpensive and sensitive method offers a quick, reliable way to measure lactate levels in samples like yogurt.

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Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

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Published on: September 19, 2017

Area of Science:

Biomedical Engineering
Analytical Chemistry
Biosensors

Background:

Lactate measurement is crucial in various fields, including clinical diagnostics and food analysis.
Existing methods for lactate detection can be time-consuming or require complex instrumentation.

Purpose of the Study:

To develop a novel, sensitive, and rapid chemiluminescence-based one-shot biosensor for lactate determination.
To optimize the biosensor's membrane composition and reaction conditions for enhanced performance.

Main Methods:

Immobilization of lactate oxidase (LOx), luminol, Arthromyces ramosus peroxidase (ARP), and metallic aluminum in a polyion complex membrane.
Chemiluminescence detection using a luminometer upon sample injection.
Optimization of membrane composition and reaction parameters.

Main Results:

The one-shot biosensor demonstrated rapid response (2 min CL acquisition time).
Achieved a wide dynamic range (5 x 10(-5) to 4 x 10(-3) M) with a low detection limit (9.2 x 10(-6) M).
Exhibited good sensor-to-sensor reproducibility (5.5% R.S.D.) and was validated for lactate analysis in yogurt.

Conclusions:

The developed chemiluminescence biosensor offers a quick, inexpensive, and sensitive method for lactate analysis.
The one-shot design and use of conventional instrumentation make it a practical tool.
Validated results in real-world samples (yogurt) confirm its applicability.