Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Microbial Biosensors

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Machine Learning Applied to Electrochemical Data Processing for Improved Analyte Quantification in Complex Saliva.

Electroanalysis·2026

Same author

Heat-actuated valve implementation in a point-of-care, paper-based microfluidic device for infectious disease detection.

PloS one·2026

Same author

Progress on Electrochemical Sensing of Pharmaceutical Drugs in Complex Biofluids.

Chemosensors (Basel, Switzerland)·2026

Same author

A low-cost point-of-care device for the simultaneous detection of two sexually transmitted bacterial pathogens in vaginal swab samples.

The Analyst·2025

Same author

Field‑use device for the electrochemical quantification of carbamazepine levels in a background of human saliva.

Journal of applied electrochemistry·2025

Same author

High-sensitivity detection of <i>Mycobacterium tuberculosis</i> DNA in tongue swab samples.

Journal of clinical microbiology·2025

Same journal

Tunable self-assembling cellular microarray for single-neutrophil vital and suicidal extracellular traps.

Lab on a chip·2026

Same journal

Precise programmable tumor cell subpopulation sorting <i>via</i> an electromagnetic microfluidic platform.

Lab on a chip·2026

Same journal

Bridging dimensions: combining one- and two-photon 3D printing for microfluidic device fabrication.

Lab on a chip·2026

Same journal

Microfluidic rare cell analysis beyond counting: workflow design from enrichment to multi-omics.

Lab on a chip·2026

Same journal

A sperm racetrack to separate sperm by swim speed.

Lab on a chip·2026

Same journal

Controlled encapsulation and droplet size prediction in two-step microfluidic double emulsions.

Lab on a chip·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 28, 2026

Use of a High-throughput In Vitro Microfluidic System to Develop Oral Multi-species Biofilms

Use of a High-throughput In Vitro Microfluidic System to Develop Oral Multi-species Biofilms

Published on: December 1, 2014

Conditioning saliva for use in a microfluidic biosensor.

Kristen L Helton¹, Kjell E Nelson, Elain Fu

¹Department of Bioengineering, University of Washington, Seattle, WA 98195, USA.

|October 23, 2008

Summary

This summary is machine-generated.

Saliva sample conditioning using filtration and H-filter extraction reduces fouling of biosensor surfaces. This method enhances compatibility of raw patient saliva for microfluidic immunoassays.

More Related Videos

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Published on: September 19, 2017

Systematic Approach to Identify Novel Antimicrobial and Antibiofilm Molecules from Plants' Extracts and Fractions to Prevent Dental Caries

Systematic Approach to Identify Novel Antimicrobial and Antibiofilm Molecules from Plants' Extracts and Fractions to Prevent Dental Caries

Published on: March 31, 2021

Related Experiment Videos

Last Updated: Jun 28, 2026

Use of a High-throughput In Vitro Microfluidic System to Develop Oral Multi-species Biofilms

Use of a High-throughput In Vitro Microfluidic System to Develop Oral Multi-species Biofilms

Published on: December 1, 2014

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Published on: September 19, 2017

Systematic Approach to Identify Novel Antimicrobial and Antibiofilm Molecules from Plants' Extracts and Fractions to Prevent Dental Caries

Systematic Approach to Identify Novel Antimicrobial and Antibiofilm Molecules from Plants' Extracts and Fractions to Prevent Dental Caries

Published on: March 31, 2021

Area of Science:

Biomedical Engineering
Analytical Chemistry
Biochemistry

Background:

Microfluidic immunoassays are sensitive to sample matrix effects, particularly biofouling from raw saliva.
Components like biosensor surfaces are prone to fouling by proteins and glycoproteins in saliva.
Effective saliva sample preparation is crucial for reliable point-of-care diagnostics.

Purpose of the Study:

To develop and validate a saliva conditioning method for microfluidic immunoassay compatibility.
To reduce biosensor surface fouling caused by salivary components.
To preserve analyte integrity during sample preparation.

Main Methods:

Stimulated whole human saliva was filtered to remove cells, debris, and high molecular weight glycoproteins (mucins).
An H-filter was employed to separate a small molecule analyte (phenytoin) from remaining large molecular weight species.
The conditioned saliva was analyzed for analyte recovery and reduction in fouling components.

Main Results:

Filtration and H-filter extraction reduced glycoproteins by 97% and proteins by 92%.
The method retained 23% of the target analyte (phenytoin).
Surface fouling was reduced 3.6-fold compared to untreated or filtered saliva alone.

Conclusions:

This two-step saliva conditioning approach significantly reduces biofouling on biosensor surfaces.
The method enables the use of fouling-sensitive detection techniques with clinical saliva samples.
Optimized sample preparation is key for advancing saliva-based microfluidic diagnostics.