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Related Concept Videos

Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...

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Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis
10:38

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis

Published on: September 3, 2013

A continuous size-dependent particle separator using a negative dielectrophoretic virtual pillar array.

Sunghwan Chang1, Young-Ho Cho

  • 1Department of Bio and Brain Engineering, Daejeon 305-701, Republic of Korea.

Lab on a Chip
|October 23, 2008
PubMed
Summary

This study introduces a novel particle separator that uses negative dielectrophoretic (DEP) virtual pillars to achieve continuous, size-dependent particle separation without clogging. The system

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Spatial Separation of Molecular Conformers and Clusters
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Spatial Separation of Molecular Conformers and Clusters

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Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis
10:38

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis

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Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow
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Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

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Spatial Separation of Molecular Conformers and Clusters
10:37

Spatial Separation of Molecular Conformers and Clusters

Published on: January 9, 2014

Area of Science:

  • Biotechnology
  • Microfluidics
  • Biophysics

Background:

  • Traditional particle separators face challenges with particle clogging and fixed size separation ranges.
  • Mechanical sieving structures are prone to clogging, limiting their practical application.
  • Existing methods often lack flexibility in adjusting the range of separable particle sizes.

Purpose of the Study:

  • To develop a continuous, size-dependent particle separator that overcomes clogging and allows for tunable separation parameters.
  • To demonstrate the efficacy of negative dielectrophoretic (DEP) virtual pillars for particle separation.
  • To enable clog-free and adaptable particle separation for various applications.

Main Methods:

  • Utilized a negative dielectrophoretic (DEP) virtual pillar array generated by DEP forces.
  • Employed a sinusoidal wave at 500 kHz with varying root mean square (rms) voltages (10 Vrms and 8 Vrms).
  • Operated at different flow rates (0.40 µL/min and 0.11 µL/min) to test separation of polystyrene beads and blood cells.

Main Results:

  • Achieved clog-free separation of polystyrene beads (5.7-11.9 µm) with high purity (>99%) at optimized voltage and flow rate.
  • Successfully separated red blood cells (5.4 µm) and white blood cells (8.1 µm) with >99% purity.
  • Demonstrated tunable separation by adjusting applied voltage, overcoming limitations of fixed-size separators.

Conclusions:

  • The developed negative DEP virtual pillar array offers a clog-free solution for size-dependent particle separation.
  • The system allows for dynamic adjustment of separable particle sizes, enhancing its versatility.
  • This technology holds promise for applications requiring precise and adaptable cell and particle separation.