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Published on: July 3, 2019
[The protein oxidative damage level and lifespan modulation by xenobiotics in Drosophila melanogaster]
Abstract:
The effect of xenobiotics on protein oxidative damage and lifespan of Drosophila melanogaster Meig., line Oregon-R, was studied. Addition of the uncoupler 2,4-dinitrophenol (DNP) to the nutritional mixture results in an induction of synthesis of heat shock proteins and increase of lifespan of insects, whereas sodium nitroprusside (SNP) has negative effect on flies viability connected, probably, with activation of processes of proteins oxidative damage. It is shown that DNP essentially corrects the SNP negative action on insects' survival rates and this "normalizing" action is revealed both at a level of sensitivity of flies to exogenic stresses and protein carbonils level and at a level of insects lifespan as a whole. It is supposed that DNP protects from SNP negative action on flies viability by reduction of intensity of free radicals production and/or induction of heat shock proteins synthesis. Consequence of that is reduction of oxidative proteins damage degree and increase of survival rate (life span) of flies.
Insights
The uncoupler 2,4-dinitrophenol (DNP) extends fruit fly lifespan and protects against oxidative stress. DNP counteracts the negative effects of sodium nitroprusside (SNP), improving survival rates.
Area of Science:
- Toxicology
- Biochemistry
- Gerontology
Context:
- Xenobiotics, environmental chemicals, can induce oxidative stress and impact lifespan.
- Protein oxidative damage is a key factor in aging and disease.
- Drosophila melanogaster serves as a model organism for studying aging and stress responses.
Purpose:
- To investigate the effects of 2,4-dinitrophenol (DNP) and sodium nitroprusside (SNP) on protein oxidative damage and lifespan in Drosophila melanogaster.
- To determine if DNP can mitigate the negative effects of SNP on fly viability and longevity.
Summary:
- Administration of DNP increased heat shock protein synthesis and extended lifespan in fruit flies.
- SNP exposure negatively affected fly viability, likely due to increased protein oxidative damage.
- DNP significantly counteracted the detrimental effects of SNP, improving survival rates and reducing protein carbonylation.
Impact:
- DNP's protective mechanism against SNP involves reducing free radical production and/or inducing heat shock proteins.
- This study highlights DNP's potential role in mitigating xenobiotic-induced oxidative stress and extending lifespan.
- Findings contribute to understanding oxidative stress, aging, and potential interventions in model organisms.
