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Related Concept Videos

Subviral Agents01:29

Subviral Agents

Subviral agents are infectious entities that resemble viruses but lack one or more viral components, such as a capsid or essential replication machinery. These agents include viroids, prions, and satellites, each possessing distinct structural and functional characteristics that influence their mode of infection and replication.Viroids are the simplest subviral agents, consisting of circular, single-stranded RNA molecules without a protein coat. They exclusively infect plants, relying entirely...

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Related Experiment Video

Updated: Jun 28, 2026

Direct Agroinoculation of Maize Seedlings by Injection with Recombinant Foxtail Mosaic Virus and Sugarcane Mosaic Virus Infectious Clones
05:56

Direct Agroinoculation of Maize Seedlings by Injection with Recombinant Foxtail Mosaic Virus and Sugarcane Mosaic Virus Infectious Clones

Published on: February 27, 2021

Variability among turnip mosaic potyvirus isolates.

L Stavolone, D Alioto, A Ragozzino

    Phytopathology
    |October 24, 2008
    PubMed
    Summary
    This summary is machine-generated.

    Turnip mosaic virus (TuMV) isolates from Italy show significant genetic and biological variation. Even isolates with similar pathotypes can differ in host range, symptoms, and serology, highlighting complex viral diversity.

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    Last Updated: Jun 28, 2026

    Direct Agroinoculation of Maize Seedlings by Injection with Recombinant Foxtail Mosaic Virus and Sugarcane Mosaic Virus Infectious Clones
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    Direct Agroinoculation of Maize Seedlings by Injection with Recombinant Foxtail Mosaic Virus and Sugarcane Mosaic Virus Infectious Clones

    Published on: February 27, 2021

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    Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
    08:56

    Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes

    Published on: July 27, 2018

    Area of Science:

    • Plant virology
    • Molecular biology
    • Agricultural science

    Background:

    • Turnip mosaic virus (TuMV) is a significant pathogen affecting cruciferous crops.
    • Understanding the genetic diversity of TuMV is crucial for effective disease management.

    Purpose of the Study:

    • To characterize eight TuMV isolates from Italy.
    • To assess the biological and molecular variability among these isolates.
    • To evaluate diagnostic methods for differentiating TuMV strains.

    Main Methods:

    • Host range and symptomatology studies.
    • Serological differentiation using monoclonal antibodies against the coat protein.
    • Single-strand conformation polymorphism (SSCP) analysis of the coat protein gene.

    Main Results:

    • Significant biological and genetic variation was observed among the eight TuMV isolates.
    • Monoclonal antibodies and SSCP analysis effectively differentiated most isolates.
    • Isolates ITA1 and ITA3 were indistinguishable by both serological and SSCP methods.
    • Pathotype determination using differential hosts varied across environments.

    Conclusions:

    • Considerable TuMV diversity exists even within a small geographical area.
    • Pathotype classification can be inconsistent and requires complementary diagnostic tools.
    • Monoclonal antibodies and SSCP are reliable methods for TuMV isolate differentiation.
    • Further research is needed to understand the implications of TuMV variability on crop disease.