[Iso-octane soluble complex of cytochrome c with bovine brain phospholipids]

The conditions are determined for formation of the isooctane-soluble complex of cytochrome c with bull brain phospholipids. Molecular ratio protein/lipid in lipocytochrome c is 1 : 20 divided by 30. Ferri- and ferroforms of protein have different affinity to phospholipids. The ferrocytochrome c in the complex is subjected to self-oxidation in the absence of redox agents. Interaction of protein with phospholipids in water phase is shown to be of electrostatic nature. The cytochrome c conformation in water solution and in the complex does not differ significantly. But the mode of packing of lipid and protein molecules in the isooctane-soluble complex depends upon the degree of oxidation as has been shown by sedimentation methods. Cytochrome c maintains his conformation in a complex with phospholipids and retains the ability to redox reactions.