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Amplification of rat microsatellite loci in Mastomys coucha Smith, 1836
1Department of Animal Science, M.J.P. Rohilkhand University, Bareilly 2430 06, India.
Abstract:
The multimammate rat M. coucha is the most widespread strain to be introduced in biomedical research and various stocks of this strain are maintained in laboratories across the globe. It is an ideal carrier of normally non-human disease to the domestic environment. In order to analyze genetic purity, strains of M. coucha were subjected to PCR-based DNA fingerprinting using sequence tagged microsatellite markers to evolve molecular signature to them. For this, 10 rats sequenced tagged microsatellite markers were used to investigate for their applicability of cross-species amplification in the genome of M. coucha. Out of 10 microsatellite primers tested, four (40%) microsatellite primer pairs [Carboxypeptidase B (CBP), Calmodulin (CALM3), Cell surface protein (CSPMO2) and Insulin like growth factor-I (IGF-1)] could be amplified successfully with exact with product size of 159, 145, 186 and 203 bps respectively in rat. The results suggest that since the above mentioned microsatellite primers get amplified successfully in M. coucha, they may be useful for genetic characterization, evaluation, strain improvement and biomedical research.
Insights
Genetic purity of the multimammate rat (M. coucha) was analyzed using DNA fingerprinting. Four microsatellite primer pairs were successfully amplified, enabling molecular signatures for M. coucha in research.
Area of Science:
- Genetics
- Molecular Biology
- Zoology
Background:
- The multimammate rat (M. coucha) is a widely used model organism in biomedical research.
- Maintaining genetic purity of M. coucha strains is crucial for reliable research outcomes.
- M. coucha can act as a carrier for diseases transmissible from non-human to domestic environments.
Purpose of the Study:
- To develop molecular signatures for M. coucha strains.
- To assess the genetic purity of M. coucha.
- To identify suitable microsatellite markers for M. coucha genetic characterization.
Main Methods:
- Polymerase Chain Reaction (PCR)-based DNA fingerprinting was employed.
- Ten sequence-tagged microsatellite markers were tested for cross-species amplification in M. coucha.
- Successful amplification was confirmed by product size.
Main Results:
- Four out of ten microsatellite primer pairs showed successful amplification in M. coucha.
- The specific primer pairs amplifying successfully were Carboxypeptidase B (CBP), Calmodulin (CALM3), Cell surface protein (CSPMO2), and Insulin-like growth factor-I (IGF-1).
- The amplified product sizes were 159 bp (CBP), 145 bp (CALM3), 186 bp (CSPMO2), and 203 bp (IGF-1).
Conclusions:
- The identified microsatellite markers are effective for genetic characterization of M. coucha.
- These markers can aid in evaluating and improving M. coucha strains for biomedical research.
- Establishing molecular signatures is vital for ensuring the integrity of M. coucha in research settings.
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