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Silica supported phospholipid layers doped with GM1: A comparison between different methods
Olga Santos1, Thomas Arnebrant
1Biomedical Laboratory Science & Biomedical Technology, Faculty of Health and Society, Malmö University, SE-20506 Malmö, Sweden. olgasantos@hotmail.com
Journal of Colloid and Interface Science
|October 28, 2008
Summary
Vesicle fusion is the most effective method for creating stable lipid layers (DOPC:GM1) on silica and polystyrene surfaces, crucial for developing new ELISA assays. This technique ensures lipid molecule stability against detergent washings.
Area of Science:
- Biomaterials Science
- Surface Chemistry
- Assay Development
Background:
- Developing stable lipid coatings on surfaces is essential for advanced diagnostic assays like ELISA.
- Existing methods struggle to create reproducible lipid layers resistant to detergent washing.
Purpose of the Study:
- To evaluate different methods for creating stable 1,2-dioleolyl-sn-glycero-3-phosphocholine (DOPC) and monosialoganglioside (GM1) lipid layers on silica and polystyrene surfaces.
- To identify a reproducible method for coating surfaces with lipid molecules resistant to detergent washings for improved ELISA development.
Main Methods:
- Co-adsorption from mixed micellar solutions (DOPC:GM1 with n-dodecyl-beta-D-maltoside).
- Vesicle fusion of DOPC:GM1 liposomes onto surfaces.
- Deposition of GM1 from organic solvent followed by hydration.
- Cholera toxin subunit B (CTB) was used to detect GM1 presence and quantify adsorption.
Main Results:
- Vesicle fusion successfully created stable mono- and bilayers on silica and polystyrene surfaces.
- Mixed micellar solution method yielded unstable GM1-doped layers, while the hydration method showed partial lipid loss.
- CTB adsorption correlated with GM1 concentration, with bilayers showing higher adsorption than monolayers.
Conclusions:
- Vesicle fusion is the optimal method for fabricating robust and reproducible lipid layers for biosensor applications.
- The stability of lipid layers created by vesicle fusion is suitable for applications requiring resistance to surfactant washing, such as ELISA.
- The study provides a reliable method for surface functionalization with lipids, enhancing diagnostic assay development.
