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DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

DNA Isolation

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Updated: Jun 28, 2026

Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains

Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains

Published on: November 30, 2021

Developing a simple method to process bone samples prior to DNA isolation.

Richard Li¹, Sandra Chapman, Mary Thompson

¹Department of Science, John Jay College of Criminal Justice, The City University of New York, New York, NY 10019, USA. rli@jjay.cuny.edu

Legal Medicine (Tokyo, Japan)

|October 28, 2008

Summary

This summary is machine-generated.

A new trypsin-based method simplifies bone processing for DNA extraction, reducing labor and time. This technique aids in automated human identification from bone samples.

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Last Updated: Jun 28, 2026

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Murine Hind Limb Long Bone Dissection and Bone Marrow Isolation

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Published on: April 14, 2016

Area of Science:

Forensic Science
Molecular Biology
Biochemistry

Background:

Bone tissue is a valuable source for DNA recovery in human identification.
Current methods for bone processing are labor-intensive and time-consuming, hindering automation.
Physical methods like sanding are often required for cleaning bone specimens.

Purpose of the Study:

To develop a simplified, automatable method for bone processing prior to DNA isolation.
To reduce the labor and time associated with preparing bone samples for DNA analysis.
To introduce a trypsin-based procedure as an alternative to physical cleaning methods.

Main Methods:

A novel method utilizing trypsin treatment was developed for bone sample processing.
Bone fragments were incubated with a trypsin solution to remove soft tissue and outer surfaces.
The processed bone fragments were then used for DNA isolation.

Main Results:

The trypsin treatment effectively removed soft tissue and the outer bone surface.
This method significantly reduces the labor required compared to physical methods.
The processed bone fragments are suitable for subsequent DNA isolation.

Conclusions:

Trypsin treatment offers a simpler and more efficient approach to bone processing for DNA analysis.
This method has the potential to facilitate automation in forensic DNA profiling.
The procedure streamlines sample preparation, making human identification from bone more accessible.