Related Experiment Video
Updated: Jun 28, 2026

13:39
Optical Trapping of Nanoparticles
Published on: January 15, 2013
Small particle reagents: development of fluorescent variants
O P Jasuja1, Gagan Deep Singh, G S Sodhi
1Forensic Science, Punjabi University, Patiala-147 002, India. opjasuja@gmail.com
Science & Justice : Journal of the Forensic Science Society
|October 29, 2008
Summary
New fluorescent small particle reagents (SPR) using zinc carbonate and dyes enhance latent fingerprint development on non-porous surfaces. These reproducible SPRs offer acceptable shelf life for forensic casework.
Area of Science:
- Forensic Science
- Chemistry
- Materials Science
Background:
- Small particle reagent (SPR) is crucial for developing latent fingerprints on various surfaces.
- Molybdenum disulfide has been the conventional material for SPR, with some alternatives explored.
- Fluorescent SPRs offer potential advantages in visualization.
Purpose of the Study:
- To develop novel fluorescent small particle reagents (SPR) based on zinc carbonate.
- To evaluate the efficacy of these new SPRs for latent fingerprint development.
- To assess the reproducibility, shelf life, and optimal application parameters of the developed reagents.
Main Methods:
- Synthesis of several SPR formulations combining zinc carbonate with different fluorescent dyes.
- Application of SPRs to develop latent fingerprints on non-porous surfaces.
- Evaluation of fingerprint quality, reproducibility, and shelf life.
- Investigation of the effect of substrate immersion time on reagent performance.
Main Results:
- Successful development of multiple zinc carbonate-based fluorescent SPRs.
- High-quality and reproducible latent fingerprints were obtained on non-porous surfaces.
- The developed SPRs demonstrated acceptable shelf life for practical forensic applications.
- The relationship between immersion period and reagent effectiveness was examined.
Conclusions:
- Zinc carbonate-based fluorescent SPRs are effective for latent fingerprint development.
- These novel reagents offer a viable alternative to conventional methods.
- The findings support the use of these SPRs in forensic casework for enhanced fingerprint visualization.
Related Concept Videos
Protein Dynamics in Living Cells
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Photoluminescence: Applications
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
Super-resolution Fluorescence Microscopy
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

