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Updated: Jul 31, 2026
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Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines
Published on: November 8, 2011
Moloney leukemia virus immortalizes B lymphocytes in vitro
J Runnels1, L Serunian, M Thursby
1Department of Pathology, Tufts University School of Medicine, Boston, Massachusetts 02111.
Journal of Virology
|October 1, 1991
Summary
Researchers developed a new in vitro culture system using Moloney murine leukemia virus to immortalize B lymphocytes. This system offers a novel model for studying retroviral transformation without oncogenes.
Area of Science:
- Virology
- Immunology
- Cell Biology
Background:
- Retroviruses lacking oncogenes can induce cellular transformation.
- Studying this process requires appropriate in vitro models.
- Mature B lymphocytes are key immune cells susceptible to transformation.
Purpose of the Study:
- To develop a novel in vitro culture system for studying retroviral transformation.
- To utilize Moloney murine leukemia virus for B lymphocyte immortalization.
- To establish a model for investigating oncogene-independent transformation.
Main Methods:
- Development of an in vitro culture system.
- Induction of B lymphocyte immortalization using Moloney murine leukemia virus.
- Characterization of derived cell lines for tumorigenicity and viral dependence.
Main Results:
- Successful development of an in vitro culture system.
- Generated immortalized B lymphocyte cell lines.
- Derived cell lines were nontumorigenic.
- Sustaining these cell lines did not require continuous virus production.
Conclusions:
- A new in vitro model for studying retroviral transformation has been established.
- This model system is suitable for investigating oncogene-independent transformation processes.
- The developed cell lines provide a valuable tool for B lymphocyte research.
