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Cytotoxic T Cells-mediated Immune Response01:27

Cytotoxic T Cells-mediated Immune Response

Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
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Updated: Jun 28, 2026

Flow Cytometry-based Assay for the Monitoring of NK Cell Functions
08:17

Flow Cytometry-based Assay for the Monitoring of NK Cell Functions

Published on: October 30, 2016

Allosteric LFA-1 inhibitors modulate natural killer cell function.

Gabriele Weitz-Schmidt1, Stéphanie Chreng, Simone Riek

  • 1Department of Autoimmunity, Novartis Institutes for BioMedical Research, Basel, Switzerland. gabriele.weitz@novartis.com

Molecular Pharmacology
|October 29, 2008
PubMed
Summary
This summary is machine-generated.

Low molecular weight inhibitors of lymphocyte function-associated antigen-1 (LFA-1) effectively block natural killer (NK) cell activity. This discovery offers new therapeutic strategies for immunological diseases by targeting LFA-1 in NK cell modulation.

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Last Updated: Jun 28, 2026

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Published on: May 22, 2020

Area of Science:

  • Immunology
  • Cell Biology
  • Transplantation Science

Background:

  • Natural killer (NK) cells are crucial in immune responses, implicated in transplant rejection and autoimmune diseases.
  • Current therapeutic options lack potent methods to effectively modulate NK cell activity.

Purpose of the Study:

  • To investigate the role of lymphocyte function-associated antigen-1 (LFA-1) in NK cell effector functions.
  • To evaluate the efficacy of low molecular weight LFA-1 inhibitors in blocking NK cell activity.
  • To introduce a novel assay for monitoring NK cell activation in clinical settings.

Main Methods:

  • In vitro assessment of NK cell adhesion, activation, and cytolysis using LFA-1 inhibitors.
  • Evaluation of LFA-1 inhibition effects independent of the allosteric mechanism.
  • Development and description of a nonradioactive whole-blood assay for NK cell activation.

Main Results:

  • Low molecular weight LFA-1 inhibitors demonstrated potent blockade of NK cell adhesion, activation, and cytolysis in vitro.
  • The observed effects were consistent across different allosteric inhibition mechanisms of LFA-1.
  • A simple, nonradioactive whole-blood assay was successfully developed for monitoring NK cell activation.

Conclusions:

  • Lymphocyte function-associated antigen-1 (LFA-1) plays a critical role in NK cell effector functions.
  • Allosteric LFA-1 inhibitors represent a promising therapeutic avenue for modulating NK cell activity in immunological diseases.
  • The developed whole-blood assay is suitable for clinical monitoring of NK cell activation.