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Updated: Feb 18, 2026

In-vitro Reconstitution of Bacterial Ubiquitination and VCP/p97-mediated Elimination
Published on: January 2, 2026
A ubiquitin-like protein unleashes ubiquitin ligases
Nabiha Huq Saifee1, Ning Zheng
1Department of Pharmacology, Box 357280, University of Washington, Seattle, WA 98195, USA.
Abstract:
Modification of cullin-RING ubiquitin ligases by the ubiquitin-like molecule Nedd8 promotes substrate ubiquitination. A crystal structure of a cullin modified by Nedd8 recently reported in Cell (Duda et al., 2008) and a biochemical study in Molecular Cell (Saha and Deshaies, 2008) reveal the dramatic impact on the ligase machinery by conjugation of ubiquitin or ubiquitin-like proteins.
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