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Conducting Multiple Imaging Modes with One Fluorescence Microscope
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Exploring the limits of single emitter detection in fluorescence and extinction
1Laboratory of Physical Chemistry and opt ETH, ETH Zürich, Zürich, Switzerland.
Optics Express
|October 30, 2008
Abstract:
We present an experimental comparison and a theoretical analysis of the signal-to-noise ratios in fluorescence and extinction spectroscopy of a single emitter. We show that because of its homodyne nature the extinction measurements can be advantageous if the emitter is weakly excited. Furthermore, we discuss the potential of this method for the detection and spectroscopy of weakly emitting systems such as rare earth ions.
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