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Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences01:17

NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences

A pulse is a short burst of radio waves distributed over a range of frequencies that simultaneously excites all the nuclei in the sample. Upon passing a radio frequency pulse along the x-axis, the nuclei absorb energy corresponding to their Larmor frequencies and achieve resonance. This shifts the net magnetization vector from the z-axis toward the transverse plane. This angle of rotation of the magnetization vector, or the flip angle, is proportional to the duration and intensity of the pulse.

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Related Experiment Video

Updated: Jun 28, 2026

Low-cost Custom Fabrication and Mode-locked Operation of an All-normal-dispersion Femtosecond Fiber Laser for Multiphoton Microscopy
08:48

Low-cost Custom Fabrication and Mode-locked Operation of an All-normal-dispersion Femtosecond Fiber Laser for Multiphoton Microscopy

Published on: November 22, 2019

Nonlinear-microscopy optical-pulse sources based on mode-locked semiconductor lasers.

H Yokoyama1, A Sato, H-C Guo

  • 1New Industry Creation Hatchery Center, Tohoku University, Aoba-ku, Sendai, Japan. yoko@niche.tohoku.ac.jp

Optics Express
|October 30, 2008
PubMed
Summary
This summary is machine-generated.

We created picosecond optical-pulse sources using mode-locked semiconductor lasers for multiphoton microscopy. These lasers enable advanced bio-imaging techniques like two-photon fluorescence and harmonic generation.

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Automation of Mode Locking in a Nonlinear Polarization Rotation Fiber Laser through Output Polarization Measurements
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Related Experiment Videos

Last Updated: Jun 28, 2026

Low-cost Custom Fabrication and Mode-locked Operation of an All-normal-dispersion Femtosecond Fiber Laser for Multiphoton Microscopy
08:48

Low-cost Custom Fabrication and Mode-locked Operation of an All-normal-dispersion Femtosecond Fiber Laser for Multiphoton Microscopy

Published on: November 22, 2019

Automation of Mode Locking in a Nonlinear Polarization Rotation Fiber Laser through Output Polarization Measurements
14:18

Automation of Mode Locking in a Nonlinear Polarization Rotation Fiber Laser through Output Polarization Measurements

Published on: February 28, 2016

Area of Science:

  • Optics and Photonics
  • Biomedical Imaging
  • Laser Technology

Background:

  • Multiphoton microscopy requires specialized optical pulse sources for high-resolution bio-imaging.
  • Mode-locked semiconductor lasers offer a compact and efficient platform for generating ultrashort optical pulses.

Purpose of the Study:

  • To develop robust picosecond optical-pulse sources optimized for multiphoton microscopy applications.
  • To achieve tunable repetition rates suitable for various nonlinear optical bio-imaging modalities.

Main Methods:

  • Utilized an external-cavity geometry for stable hybrid mode-locking of semiconductor lasers at 500 MHz.
  • Employed semiconductor optical amplifiers with synchronized electric pulses to achieve subharmonic repetition rates from 1-100 MHz.
  • Incorporated two-stage Yb-doped fiber amplifiers to generate 2 ps optical pulses with kilowatt peak power at 10 MHz.

Main Results:

  • Successfully generated picosecond optical pulses with tunable repetition rates (1-100 MHz).
  • Achieved 2 ps pulse durations and kilowatt peak powers, ideal for nonlinear microscopy.
  • Demonstrated successful application in nonlinear-optic bio-imaging, including two-photon fluorescence, second-harmonic generation, and sum-frequency generation.

Conclusions:

  • Developed versatile picosecond optical-pulse sources based on mode-locked semiconductor lasers.
  • The developed sources are highly suitable for advanced multiphoton microscopy and nonlinear bio-imaging.
  • Enabled synchronized two-color pulse generation for enhanced imaging capabilities.