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Related Concept Videos

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Gas Chromatography–Mass Spectrometry (GC–MS)01:14

Gas Chromatography–Mass Spectrometry (GC–MS)

Gas chromatography–mass spectrometry (GC–MS) is the combination of analytical techniques of gas chromatography and mass spectrometry in a single instrument for analyzing a mixture of compounds. The gas chromatograph separates the compounds in the mixture, and the mass spectrometer analyzes each compound separately to determine the molecular masses and molecular structures.
A gas chromatograph consists of a long, narrow capillary column with a polysiloxane coating on the inner wall. The coating...
Optimizing Chromatographic Separations01:15

Optimizing Chromatographic Separations

Optimizing chromatographic separations is crucial for obtaining clean separations in a minimum amount of time. Optimization is required for several factors, including kinetic effects related to band broadening, plate height, capacity factor, and separation factor.
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
Sample Preparation for Analysis: Advanced Techniques01:08

Sample Preparation for Analysis: Advanced Techniques

Accurate analysis of complex samples often requires advanced preparation techniques to achieve reliable and reproducible results. Samples containing inorganic or organic materials can be challenging to dissolve or decompose effectively. Standard sample preparation methods include acid digestion, fusion, dry ashing, and wet digestion.
Acid digestion with strong acids is commonly used to dissolve inorganic materials that are insoluble (do not dissolve) in water. This method can be useful for...
Chromatography: Introduction01:10

Chromatography: Introduction

Chromatography is a technique used to separate compounds based on differences of partitioning between two phases, the stationary phase and the mobile phase.
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...

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Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis
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Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis

Published on: July 11, 2014

A separation scheme for the analysis of multicomponent samples.

J S Fritz1, G L Latwesen

  • 1Institute for Atomic Research and Department of Chemistry, Iowa State University, Ames, Iowa, USA.

Talanta
|January 1, 1970
PubMed
Summary
This summary is machine-generated.

This study presents a sequential separation scheme for analyzing 27 metal ions using chromatography and ion-exchange columns. The reliable method works for both macro and micro quantities, validated with certified reference materials.

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A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample
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A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample

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Area of Science:

  • Analytical Chemistry
  • Separation Science

Background:

  • Accurate quantification of multiple metal ions is crucial in various scientific fields.
  • Existing methods may lack the versatility to handle a wide range of metal ions simultaneously.

Purpose of the Study:

  • To develop a sequential separation scheme for the quantitative analysis of 27 different metal ions.
  • To establish a reliable method applicable to both macro and micro quantities of metals.

Main Methods:

  • Utilized a combination of five chromatographic and ion-exchange columns.
  • Employed selective eluents for sequential elution of metal ion groups.
  • Determined separated metal ions using titrimetry, flame spectrometry, and other analytical techniques.

Main Results:

  • Successfully separated and quantified 27 distinct metal ions.
  • Demonstrated the scheme's reliability through extensive testing.
  • Validated the method using U.S. National Bureau of Standards samples.

Conclusions:

  • The devised sequential separation scheme is effective for the quantitative analysis of a diverse range of metal ions.
  • The method's applicability to both macro and micro quantities broadens its utility.
  • The scheme offers a reliable approach for complex metal ion mixture analysis.