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Updated: Jun 28, 2026

Characterizing Mammalian Zinc Transporters Using an In Vitro Zinc Transport Assay
Published on: June 2, 2023
Determination of traces of zinc by fluorimetrically indicated complexometric titrations
R van Slageren1, G den Boef, W E van der Linden
1Laboratorium voor Analytische Scheikunde, Universiteit van Amsterdam, Amsterdam, The Netherlands.
Abstract:
Zinc forms fluorescent complexes with 8-hydroxyquinoline-5-sulphonic acid (HQS) in the pH region 7-10. The stability of these complexes is such that two different titration procedures are possible: the titration of zinc with HQS and the titration of zinc with EDTA in the presence of HQS. For both procedures the choice of the titration conditions is described. Also two separation procedures for zinc are described, especially suitable in connection with the EDTA titration. The practical results show that zinc in concentrations larger than 10(-6)M (0.05 mug/ml) can be titrated with good precision.
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