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Updated: Jun 28, 2026

Characterization of Biological Absorption Spectra Spanning the Visible to the Short-Wave Infrared
Published on: January 10, 2025
The error in absorption measurements, caused by the use of non-monochromatic light-II Gaussian functions
J Agterdenbos1, J Vlogtman, L van Broekhoven
1Laboratory for Analytical Chemistry, State University, Croesestraat 77a, Utrecht, Holland.
Abstract:
The errors in absorption measurements in spectrophotometry, caused by the use of non-monochromatic light, have been calculated. Gaussian functions have been assumed for the wavelength-dependence of the intensity of the incident light falling on the cell and the absorbance of the compound measured. Results found are similar to those found with parabolic functions. Experiments have been made to compare the results of the calculations with experimental values. The agreement is fairly good in most cases.
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