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Chromatographic behavior of a polyclonal antibody mixture on a strong cation exchanger column. Part II: Adsorption

Nicola Forrer1, Alessandro Butté, Massimo Morbidelli

  • 1Institute for Chemical and Bioengineering, Department of Chemistry and Applied Biosciences, ETH Zurich, 8093 Zurich, Switzerland.

Journal of Chromatography. A
|October 31, 2008
PubMed

The adsorption of a polyclonal IgG mixture on a strong cation exchanger column is characterized using a detailed multi-component pore model. This model is explicit in all transport parameters and includes salt dependent isotherms. As discussed in the first part of this work, the IgG mixture can be simplified by considering two pseudo-variants only. Linear gradient experiments are used to fit the salt dependent adsorption isotherms and the mass transport parameters for the two pseudo-variants. Using the model, breakthrough curves are predicted with good accuracy. The model is also implemented to visualize the axial and radial concentration profiles of the two pseudo-variants in the column during a loading experiment.

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