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Related Concept Videos

Spectrophotometry: Introduction01:16

Spectrophotometry: Introduction

Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
UV–Vis Spectrometers01:14

UV–Vis Spectrometers

The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...
Light as Energy01:35

Light as Energy

The energy required to carry out photosynthesis is light— typically electromagnetic radiation from the sun. The range of all possible wavelengths is known as the electromagnetic spectrum.
Photons
A photon is a discrete electromagnetic particle or bundle of energy. Photons are characterized by their frequency, wavelength, and amplitude, similar to the properties of a wave. Waves with higher frequencies transmit more energy and have shorter wavelengths than longer wavelengths that transmit less...
UV–Vis Spectroscopy: Beer–Lambert Law01:09

UV–Vis Spectroscopy: Beer–Lambert Law

The Beer-Lambert law describes the relationship between absorbance and concentration, which combines the principles established by scientists Johann Heinrich Lambert and August Beer. Lambert's law states that when light passes through a medium, the loss in intensity is directly proportional to the original intensity and the path length of the light. Beer's law proposed that the transmittance of a solution remains constant if the product of concentration and path length is constant. The modern...
UV–Vis Spectroscopy of Conjugated Systems01:32

UV–Vis Spectroscopy of Conjugated Systems

Organic compounds with conjugated double bonds show strong absorption features in the UV–visible region of the electromagnetic spectrum attributed to π → π* electronic excitations. Generally, a UV–vis absorption spectrum is recorded as a plot of absorbance vs wavelength. The wavelength of maximum absorbance, which manifests as a peak in the absorption spectrum, is denoted as λmax.
One of the factors influencing λmax is the extent of conjugation in the...
UV–Vis Spectrum01:30

UV–Vis Spectrum

When light passes through a substance, a portion of the light is absorbed while the remaining light is reflected or transmitted. If the molecule absorbs light between the wavelengths of 180–400 nm range, the UV spectrum is obtained, and if it absorbs light in the 400–780 nm wavelength range, the visible spectrum is obtained.     
The UV–Vis spectrum of a molecule is the plot of its absorbance versus wavelength. The plot is drawn by taking molar absorptivity (ε) or log ε on the y-axis (ordinate)...

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Related Experiment Video

Updated: Jun 28, 2026

Non-invasive Assay for Chlorophyll Biosynthesis Kinetics Determination during Early Stages of Arabidopsis De-etiolation
07:58

Non-invasive Assay for Chlorophyll Biosynthesis Kinetics Determination during Early Stages of Arabidopsis De-etiolation

Published on: January 12, 2024

Spectral bandwidth in plant chlorophyll determinations.

M S Cresser1, E J O'Neill

  • 1Department of Soil Science, University of Aberdeen, Aberdeen, Scotland.

Talanta
|April 1, 1980
PubMed
Summary

This study highlights the importance of spectral bandwidth in chlorophyll determination. Quantitative data are provided to enable accurate measurements even with less advanced spectrophotometers.

Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Plant Science

Background:

  • Accurate determination of chlorophylls is crucial for plant research and environmental monitoring.
  • Recent literature often overlooks the impact of spectral bandwidth on chlorophyll measurement accuracy.
  • Spectrophotometer resolution can influence the reliability of quantitative spectral analysis.

Purpose of the Study:

  • To critically evaluate the significance of spectral bandwidth effects in chlorophyll determination.
  • To provide quantitative data for accurate chlorophyll analysis.
  • To demonstrate that reliable measurements are achievable with moderately resolved spectrophotometers.

Main Methods:

  • Critical discussion of spectral bandwidth effects in spectrophotometric analysis.

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High-Throughput Analysis of Non-Photochemical Quenching in Crops Using Pulse Amplitude Modulated Chlorophyll Fluorometry

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In Vitro Reconstitution of Light-harvesting Complexes of Plants and Green Algae
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In Vitro Reconstitution of Light-harvesting Complexes of Plants and Green Algae

Published on: October 10, 2014

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Last Updated: Jun 28, 2026

Non-invasive Assay for Chlorophyll Biosynthesis Kinetics Determination during Early Stages of Arabidopsis De-etiolation
07:58

Non-invasive Assay for Chlorophyll Biosynthesis Kinetics Determination during Early Stages of Arabidopsis De-etiolation

Published on: January 12, 2024

High-Throughput Analysis of Non-Photochemical Quenching in Crops Using Pulse Amplitude Modulated Chlorophyll Fluorometry
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  • Presentation of quantitative data derived from spectrophotometric measurements.
  • Validation of methods using spectrophotometers with moderate spectral resolution.

    • Main Results:

    • Spectral bandwidth significantly impacts the accuracy of chlorophyll determination.
    • Quantitative data presented allow for precise chlorophyll measurements.
    • Spectrophotometers with moderate resolution are sufficient for reliable chlorophyll analysis when bandwidth effects are considered.

    Conclusions:

    • Ignoring spectral bandwidth effects can lead to inaccurate chlorophyll quantification.
    • The provided data enable accurate chlorophyll determination across various research settings.
    • Researchers can confidently use moderately resolved spectrophotometers for chlorophyll analysis by accounting for bandwidth effects.