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Updated: Jun 28, 2026

A Colorimetric Method for Measuring Iron Content in Plants
Published on: September 7, 2018
Estimation of iron with salicylaldehyde hydrazone
Abstract:
Iron is determined by precipitation as the reddish orange ferrous complex of salicylaldehyde hydrazone (SH) in the pH range 10.0-11.0. With ferric iron an unstable complex is formed which changes readily to the stable ferrous complex. The iron complex has a metal-ligand ratio of 1:2, contradicting the 1:3 ratio reported earlier. The reagent can be used for separation of iron from several ions.
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