Related Experiment Video
Updated: Jun 28, 2026

Metabolomic Analysis of Barley by Gas Chromatography/Mass Spectrometry
Published on: November 8, 2024
Spectrophotometric determination of methylphenobarbitone by use of orthogonal polynomials
1Faculty of Pharmacy, University of Alexandria, Pharmaceutical Analytical Chemistry Department, Alexandra, Egypt.
Abstract:
A direct spectrophotometric method for the determination of methylphenobarbitone in presence of degradation products is presented. The method is based on use of the combined polynomial method. The coefficient of the combined polynomial P(w), calculated over the wavelength range 228-272 nm at 4-nm intervals, is unaffected by the presence of any degradation products and is linearly related to the methylphenobarbitone concentration with a relative standard deviation of 0.4%. The mean recovery is 99.1 +/- 0.8%. The method can also be used for assessing the stability of methylphenobarbitone.
Related Concept Videos
¹H NMR: Complex Splitting
Splitting diagrams or splitting tree diagrams are routinely used to depict such complex couplings. While drawing splitting diagrams, the splitting with the larger coupling constant is usually applied first.
NMR Spectroscopy of Benzene Derivatives
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)
UV–Vis Spectroscopy: Woodward–Fieser Rules
Measuring Reaction Rates
Spectrophotometry: Introduction
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...

