New spectrophotometric method for determination of cephazolin

Area of Science:

• Analytical Chemistry
• Pharmaceutical Analysis

Background:

• Cephazolin is a widely used cephalosporin antibiotic.
• Accurate determination of cephazolin is crucial for quality control and therapeutic monitoring.

Purpose of the Study:

• To develop a simple, selective, and accurate spectrophotometric method for cephazolin determination.
• To utilize acid hydrolysis and measurement of a degradation product for quantification.

Main Methods:

• Acid hydrolysis of cephazolin.
• Spectrophotometric determination of 2-mercapto-5-methylthiadiazole, a hydrolysis product.
• Method validation for accuracy and selectivity.

Main Results:

• The developed method demonstrated simplicity, selectivity, and accuracy.
• Quantification was achieved by measuring the absorbance of 2-mercapto-5-methylthiadiazole.
• The method is suitable for routine analysis.

Conclusions:

• A novel spectrophotometric assay for cephazolin has been successfully developed.
• The method offers a reliable approach for quantifying cephazolin in pharmaceutical samples.
• This technique provides an efficient alternative for cephazolin analysis.