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Updated: Jun 28, 2026
Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method
Published on: December 25, 2016
Determination of ultra trace concentrations of nitrite in polluted waters and soil
A Chaube1, A K Baveja, V K Gupta
1Department of Chemistry, Ravishankar University, Raipur(M.P., 492010 India.
Abstract:
The nitrite is used to diazotize o-nitroaniline and the o-nitrophenyldiazonium chloride formed is coupled with N-(1-naphthyl)ethylenediamine dihydrochloride. The red-violet dye (absorption maxima at 545 nm) is stable and extractable into isoamyl alcohol. Beer's law is obeyed in the range 0.1-0.6 ppm nitrite in the original sample if the aqueous system is measured, and 0.025-0.15 ppm in the sample if extraction is used. The molar absorptivity is 6.04 x 10(4)l.mole(-1).cm(-1).
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