Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Mass Spectrometry: Isotope Effect

Mass Spectrometry: Isotope Effect

Most elements exist in nature as a mixture of isotopes. The isotopes differ in weight due to their respective number of neutrons. The molecular weight of a molecule is different depending on the specific isotope of its elements involved. As a result, the mass spectrum of the molecule exhibits peaks from the same fragment at multiple positions. The positions of these mass signals depend on the mass differences between isotopes. Furthermore, the intensity of these signals is dependent on the...

Atomic Emission Spectroscopy: Lab

Atomic Emission Spectroscopy: Lab

AES is a powerful analytical technique, especially effective when used with plasma sources, producing abundant spectra in characteristic emission lines. The Inductively Coupled Plasma (ICP), in particular, yields superior quantitative analytical data due to its high stability, low noise, low background, and minimal interferences under optimal experimental conditions. However, newer air-operated microwave sources are emerging as promising alternatives that could be more cost-effective than...

Mass Spectrum: Interpretation

Mass Spectrum: Interpretation

An unknown compound can be established by identifying the molecular ion peak in the mass spectrum. The molecular ion peak is often weak or absent due to the predominance of fragmentation in high-energy electron beams. In such cases, a soft-energy electron beam can be used to scan the spectrum to enhance the intensity of the molecular ion peak. Additionally, chemical ionization, field ionization, and desorption ionization spectra are used to obtain a relatively intense molecular ion peak.To...

High-Resolution Mass Spectrometry (HRMS)

High-Resolution Mass Spectrometry (HRMS)

The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For example, the mass of helium...

Atomic Spectroscopy: Absorption, Emission, and Fluorescence

Atomic Spectroscopy: Absorption, Emission, and Fluorescence

Atomic spectroscopy is a vital tool in elemental analysis, both qualitatively and quantitatively. It can be broadly divided into optical spectroscopy, mass spectroscopy, and X-ray spectroscopy methods. The optical spectroscopic methods are atomic absorption spectroscopy (AAS), atomic emission spectroscopy (AES), and atomic fluorescence spectroscopy (AFS). The first step in all three methods is atomization, where the solid, liquid, or solution-phase samples are converted into gas-phase atoms and...

¹³C NMR: ¹H–¹³C Decoupling

¹³C NMR: ¹H–¹³C Decoupling

The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Selectivity in the laser-induced photochemistry of iodine + acetylene in the gas phase.

Journal of the American Chemical Society·2012

Same author

An absorption detection approach for multiplexed capillary electrophoresis using a linear photodiode array.

Analytical chemistry·2011

Same author

People: therese m. Cotton (1939-1998).

Analytical chemistry·2011

Same author

Laser Vaporization/Ionization Interface for Capillary Electrophoresis-Time-of-Flight Mass Spectrometry.

Analytical chemistry·2011

Same author

Characterization of Nonbonded Poly(ethylene oxide) Coating for Capillary Electrophoresis via Continuous Monitoring of Electroosmotic Flow.

Analytical chemistry·2011

Same author

Two-dimensional spatial imaging based on acoustooptical deflectors.

Applied optics·2010

Search research articles

Related Experiment Video

Updated: Jun 28, 2026

Sampling and Pretreatment of Tooth Enamel Carbonate for Stable Carbon and Oxygen Isotope Analysis

Sampling and Pretreatment of Tooth Enamel Carbonate for Stable Carbon and Oxygen Isotope Analysis

Published on: August 15, 2018

Stable-isotope ratio analysis based on atomic hyperfine structure and optogalvanic spectroscopy.

W G Tong¹, E S Yeung

¹Department of Chemistry and Ames Laboratory, Iowa State University, Ames, Iowa 50011, U.S.A.

|September 1, 1984

Summary

High-resolution optogalvanic spectroscopy precisely measured atomic hyperfine structures in copper. This method accurately determined copper isotopic abundances, even at low concentrations.

More Related Videos

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis

Published on: November 17, 2011

Atom Probe Tomography Analysis of Exsolved Mineral Phases

Atom Probe Tomography Analysis of Exsolved Mineral Phases

Published on: October 25, 2019

Related Experiment Videos

Last Updated: Jun 28, 2026

Sampling and Pretreatment of Tooth Enamel Carbonate for Stable Carbon and Oxygen Isotope Analysis

Sampling and Pretreatment of Tooth Enamel Carbonate for Stable Carbon and Oxygen Isotope Analysis

Published on: August 15, 2018

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis

Published on: November 17, 2011

Atom Probe Tomography Analysis of Exsolved Mineral Phases

Atom Probe Tomography Analysis of Exsolved Mineral Phases

Published on: October 25, 2019

Area of Science:

Atomic Physics
Spectroscopy
Analytical Chemistry

Background:

Atomic hyperfine structures provide insights into nuclear properties.
Optogalvanic spectroscopy is a sensitive technique for atomic analysis.
Accurate isotopic abundance measurements are crucial for various applications.

Purpose of the Study:

To measure atomic hyperfine structures of the Cu I transition at 5782 A.
To determine the relative isotopic abundances of copper isotopes (63Cu and 65Cu).
To assess the applicability of the technique for trace copper analysis.

Main Methods:

Utilized high-resolution continuous-wave (cw) dye laser optogalvanic spectroscopy.
Employed electro-deposited copper samples on a home-made hollow-cathode lamp.
Applied spectral deconvolution techniques for data analysis.

Main Results:

Successfully measured atomic hyperfine structures for the specified Cu I transition.
Determined relative isotopic abundances of 63Cu and 65Cu with high accuracy and precision.
Demonstrated the technique's effectiveness for copper concentrations as low as 1.6 ppm.

Conclusions:

High-resolution optogalvanic spectroscopy is a viable method for studying copper hyperfine structures.
The developed technique offers precise determination of copper isotopic composition.
This method has potential applications in trace element analysis and materials science.