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Related Concept Videos

High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
High-Performance Liquid Chromatography: Instrumentation00:57

High-Performance Liquid Chromatography: Instrumentation

High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
Sample Preparation for Analysis: Overview01:21

Sample Preparation for Analysis: Overview

Sample preparation is an essential step in the analytical process. It involves preparing a sample so that it can be analyzed accurately. The goal is to extract the analyte, the substance you want to measure, from the sample while removing any components that may interfere with the analysis. Sample preparation techniques vary depending on the physical state of the sample.
Bulk or large solid samples are typically reduced in size using grinding, crushing, or milling techniques to increase the...
Sublimation01:03

Sublimation

Sublimation is the direct transformation of a solid to a gaseous state. For instance, at standard pressure and room temperature, solid carbon dioxide sublimes to gaseous carbon dioxide. The phase diagram depicts the conditions required for sublimation. This process occurs at the solid-gas phase boundary and is not observed above the triple point of the substance. The reverse of sublimation is called deposition, where a gaseous substance condenses directly into a solid. Sublimation and...
Qualitative Analysis03:46

Qualitative Analysis

For solutions containing mixtures of different cations, the identity of each cation can be determined by qualitative analysis. This technique involves a series of selective precipitations with different chemical reagents, each reaction producing a characteristic precipitate for a specific group of cations. Metal ions within a group are further separated by varying the pH, heating the mixture to redissolve a precipitate, or adding other reagents to form complex ions.
For instance, group IV...
Qualitative Analysis01:10

Qualitative Analysis

Qualitative analysis is the process of identifying elements, ions, or compounds in an unknown sample. It is the first and most fundamental type of analysis based on the hierarchy of analytical goals. This hierarchy is significant as it provides a structured approach to scientific research, with qualitative analysis serving as the initial step, providing essential information before moving on to quantitative or other forms of analysis.
There are two main approaches to qualitative analysis:...

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Related Experiment Video

Updated: Jun 28, 2026

Clean Sampling and Analysis of River and Estuarine Waters for Trace Metal Studies
10:44

Clean Sampling and Analysis of River and Estuarine Waters for Trace Metal Studies

Published on: July 1, 2016

The analysis of solid and liquid high-purity substances.

G G Devyatykh1, Y A Karpov

  • 1Institute of Chemistry, USSR Academy of Sciences, Gorky, USSR.

Talanta
|January 1, 1987
PubMed
Summary

This review covers challenges in atomic spectrometry, mass spectrometry, and activation analysis for high-purity materials. It details sample preparation, impurity preconcentration, and gas-forming trace element determination methods.

Area of Science:

  • Analytical Chemistry
  • Materials Science

Background:

  • Elemental analysis of high-purity substances is critical for advanced materials.
  • Existing methods face challenges in sensitivity and accuracy for trace impurities.

Purpose of the Study:

  • To review the limitations of common elemental analysis techniques.
  • To discuss optimized methods for sample preparation and impurity determination.

Main Methods:

  • Review of atomic spectrometry techniques.
  • Review of mass spectrometry techniques.
  • Review of activation analysis methods.

Main Results:

  • Identified challenges in elemental analysis of high-purity substances.
  • Discussed specific techniques for sample preparation and impurity preconcentration.

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Analysis of Complex Molecules and Their Reactions on Surfaces by Means of Cluster-Induced Desorption/Ionization Mass Spectrometry
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A Package of Established Analytical Tools to Investigate the Solid-State Alteration of Lipid-Based Excipients
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A Package of Established Analytical Tools to Investigate the Solid-State Alteration of Lipid-Based Excipients

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Last Updated: Jun 28, 2026

Clean Sampling and Analysis of River and Estuarine Waters for Trace Metal Studies
10:44

Clean Sampling and Analysis of River and Estuarine Waters for Trace Metal Studies

Published on: July 1, 2016

Analysis of Complex Molecules and Their Reactions on Surfaces by Means of Cluster-Induced Desorption/Ionization Mass Spectrometry
07:53

Analysis of Complex Molecules and Their Reactions on Surfaces by Means of Cluster-Induced Desorption/Ionization Mass Spectrometry

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A Package of Established Analytical Tools to Investigate the Solid-State Alteration of Lipid-Based Excipients
11:27

A Package of Established Analytical Tools to Investigate the Solid-State Alteration of Lipid-Based Excipients

Published on: August 9, 2022

  • Highlighted methods for determining gas-forming trace elements.
  • Conclusions:

    • Optimized sample preparation and preconcentration are key for accurate analysis.
    • Advanced spectrometry and activation methods are essential for high-purity materials.