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Updated: Jun 28, 2026
05:51
Direct Detection of the Acetate-forming Activity of the Enzyme Acetate Kinase
Published on: December 19, 2011
Assay of enzyme effectors.
I F Dolmanova1, T N Shekhovtsova, V V Kutcheryaeva
1Lomonosov Moscow State University, Moscow, USSR.
Talanta
|January 1, 1987
Summary
This study investigated how organic compounds and metal ions affect enzyme activity. New enzymic methods were developed for sensitive detection of sulfur, nitrogen, mercury, and lead.
Area of Science:
- Biochemistry
- Analytical Chemistry
Background:
- Enzyme kinetics are crucial for understanding biological processes.
- Horseradish peroxidase and alkaline phosphatase are important enzymes with various applications.
- Accurate quantification of environmental contaminants is essential.
Purpose of the Study:
- To investigate the impact of organic compounds and metal ions on horseradish peroxidase and alkaline phosphatase activity.
- To develop sensitive enzymic methods for determining sulfur, nitrogen, mercury, and lead concentrations.
Main Methods:
- Studied the effects of various organic compounds and metal ions on enzyme catalysis.
- Developed and applied enzymic assays for quantitative analysis.
- Utilized spectrophotometric detection for reaction monitoring.
Main Results:
- Identified specific organic compounds and metal ions that modulate enzyme activity.
- Established detection limits for sulfur (10^-5-10^-4 M), nitrogen (2x10^-7-3x10^-5 M), mercury (3x10^-7 μg/ml), and lead (6x10^-4 μg/ml).
Conclusions:
- Enzyme activity is significantly influenced by the presence of certain organic compounds and metal ions.
- The developed enzymic methods offer sensitive and specific quantification of target analytes.
- These findings contribute to a better understanding of enzyme inhibition/activation and environmental monitoring.
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