Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
Fluid Mosaic Model01:34

Fluid Mosaic Model

The fluid mosaic model was first proposed as a visual representation of research observations. The model comprises the composition and dynamics of membranes and serves as a foundation for future membrane-related studies. The model depicts the structure of the plasma membrane with a variety of components, which include phospholipids, proteins, and carbohydrates. These integral molecules are loosely bound, defining the cell’s border and providing fluidity for optimal function.LipidsThe most...
Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Phosphorimetry.

Journal of research of the National Bureau of Standards. Section A, Physics and chemistry·2021
Same author

Flame spectrometry.

Analytical chemistry·2012
Same author

Rapid atomic absorption determination of silver and copper by sequential atomization from a graphite rod.

Analytical chemistry·2012
Same author

New, simple windowless cell for front-surface fluorometry.

Analytical chemistry·2012
Same author

Double modulation-optical scanning and mechanical chopping-in atomic absorption spectrometry using a continuum source.

Analytical chemistry·2012
Same author

Micro-organization in dissolved humic acids.

Environmental science & technology·2011
Same journal

Electrochemical determination of Azelastine using a Calix[4]arene-doped polyaniline screen-printed sensor in pharmaceutical and biological samples.

Talanta·2026
Same journal

Electrical detection of cisplatin-DNA adduct formation using dielectrophoresis-based impedance spectroscopy.

Talanta·2026
Same journal

A dual-response near-infrared fluorescent probe for hypochlorite and viscosity detection in ferroptosis and rheumatoid arthritis model.

Talanta·2026
Same journal

Rapid and real-time detection of trace BPA based on solution-gated graphene field-effect transistor by cerium-doped ZIF-8 carbon material.

Talanta·2026
Same journal

Resolution of chiral drug cocrystals: complementary roles of NMR, low-frequency Raman and vibrational circular dichroism.

Talanta·2026
Same journal

Conversion of food waste to biochar via pyrolysis: Production processes, material properties, and environmental applications.

Talanta·2026
See all related articles

Related Experiment Video

Updated: Jun 28, 2026

Fluorescence detection methods for microfluidic droplet platforms
14:16

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

Fluorescence in thin liquid films: a simple model.

R von Wandruszka1, J D Winefordner

  • 1Department of Chemistry, University of Florida, Gainesville, Florida 32611, USA.

Talanta
|June 1, 1987
PubMed
Summary
This summary is machine-generated.

A new model explains how film drainage affects fluorescent dye properties. This research is crucial for understanding thin-film behavior and optical characteristics.

More Related Videos

Fluorescence Recovery after Merging a Droplet to Measure the Two-dimensional Diffusion of a Phospholipid Monolayer
07:54

Fluorescence Recovery after Merging a Droplet to Measure the Two-dimensional Diffusion of a Phospholipid Monolayer

Published on: October 15, 2015

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope
15:10

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope

Published on: October 9, 2014

Related Experiment Videos

Last Updated: Jun 28, 2026

Fluorescence detection methods for microfluidic droplet platforms
14:16

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

Fluorescence Recovery after Merging a Droplet to Measure the Two-dimensional Diffusion of a Phospholipid Monolayer
07:54

Fluorescence Recovery after Merging a Droplet to Measure the Two-dimensional Diffusion of a Phospholipid Monolayer

Published on: October 15, 2015

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope
15:10

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope

Published on: October 9, 2014

Area of Science:

  • Physical Chemistry
  • Materials Science

Background:

  • Thin films are essential in various optical and electronic applications.
  • Understanding the behavior of dyes within these films is critical for device performance.

Purpose of the Study:

  • To develop a predictive model for the fluorescence characteristics of dyes in thin films.
  • To investigate the influence of film drainage on these optical properties.

Main Methods:

  • Theoretical modeling was employed to simulate the drainage process.
  • The model incorporates key parameters affecting fluorescence.

Main Results:

  • The proposed model successfully accounts for the impact of various parameters on dye fluorescence.
  • Key parameters influencing fluorescence during drainage were identified.

Conclusions:

  • The model provides a framework for predicting and controlling dye fluorescence in dynamic thin-film systems.
  • This work has implications for the design and optimization of optoelectronic devices utilizing fluorescent thin films.