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Updated: Jun 28, 2026
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A Generalized Method for Determining Free Soluble Phenolic Acid Composition and Antioxidant Capacity of Cereals and Legumes
Published on: June 10, 2022
HPLC analysis of antioxidants
C Grosset1, D Cantin, A Villet
1Laboratoire de Chimie Analytique et Bromatologie, U.F.R. de Pharmacie de Grenoble, Domaine de la Merci, 38700 La Tronche, France.
Talanta
|March 1, 1990
Summary
This study presents new HPLC methods for analyzing antioxidant mixtures. The methods offer high sensitivity for detecting various antioxidants, crucial for quality control and research.
Area of Science:
- Analytical Chemistry
- Food Chemistry
- Pharmacology
Background:
- Antioxidants are vital compounds in food and pharmaceuticals.
- Accurate analytical methods are essential for quality control and safety.
- Existing methods may lack the sensitivity or specificity for complex mixtures.
Purpose of the Study:
- To develop and validate qualitative and quantitative High-Performance Liquid Chromatography (HPLC) methods.
- To analyze mixtures containing twelve different antioxidants.
- To establish sensitive detection limits for individual antioxidants.
Main Methods:
- Qualitative analysis using gradient elution on a C(18) column with UV detection.
- Quantitative analysis employing sequential UV and electrochemical detection.
- Isocratic elution with specific water-methanol mixtures and lithium perchlorate.
- Optimization of applied potential for electrochemical detection (+0.8 to +1.7 V).
Main Results:
- Successful separation and identification of most antioxidants using gradient elution.
- A limitation was the co-elution of propyl gallate and methyl p-hydroxybenzoate.
- Highly sensitive detection limits achieved: as low as 61 pg (UV) and 360 pg (electrochemistry).
Conclusions:
- The developed HPLC methods provide a robust approach for antioxidant analysis.
- The combination of UV and electrochemical detection enhances sensitivity and scope.
- These methods are suitable for the precise quantification of antioxidants in various matrices.
