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Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing

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Amperometric biosensors for glucose based on carbon paste modified electrodes.

A Amine¹, J M Kauffmann, G J Patriarche

¹Université Libre de Bruxelles, Institut de Pharmacie, Campus Plaine, CP 205/6, Bd. du Triomphe, 1050 Bruxelles, Belgium.

|January 1, 1991

Summary

This study details novel carbon-paste electrodes modified with glucose oxidase (GOD) for amperometric glucose sensing. These sensors offer extended linear ranges and rapid responses, proving effective for plasma glucose assays.

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Area of Science:

Electrochemistry
Biosensors
Enzyme immobilization

Background:

Carbon-paste electrodes are widely used in electrochemical sensing.
Glucose oxidase (GOD) is a key enzyme for glucose detection.
Mediators can enhance the performance of enzyme-based biosensors.

Purpose of the Study:

To develop and characterize carbon-paste electrodes modified with glucose oxidase (GOD).
To evaluate the performance of these electrodes as amperometric glucose sensors, with and without a mediator.
To assess the suitability of the developed sensors for plasma glucose determination.

Main Methods:

Modification of carbon-paste electrodes by incorporating glucose oxidase (GOD).
Amperometric detection of glucose in the presence and absence of 1,1'-dimethylferrocene mediator.
Calibration studies to determine linear ranges and response times.
Correlation of sensor results with spectrophotometric methods for plasma glucose assays.

Main Results:

The GOD-modified carbon-paste electrodes exhibited extended linear calibration ranges up to 90 mM (without mediator) and 50 mM (with mediator).
Rapid electrode responses were observed, reaching steady-state values within 30-40 seconds.
Plasma glucose assays using the developed sensor showed good correlation (r = 0.973) with spectrophotometric determinations.

Conclusions:

The GOD-modified carbon-paste electrode formulation offers advantages for amperometric glucose sensing.
The developed sensors provide a reliable and efficient method for glucose determination.
The formulation demonstrates potential for clinical applications in glucose monitoring.