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Updated: Jun 28, 2026

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
Published on: February 16, 2022
Determination of nitric acid concentration by using europium ion fluorescence
J C Coria-Garcia1, T M Niemczyk
1Department of Chemistry, University of New Mexico, Albuquerque, NM 87131, USA.
Abstract:
A method is presented for the determination of 2-10M nitric acid based on the fact that the presence of high nitric acid concentration causes an enhancement of the intensity of some bands in the fluorescence spectra of rare earth ions in solution. The 616-nm band of Eu(3+) shows the most dramatic intensity enhancement with increasing nitric acid concentration. The present method, based on the measurement of the ratio of Eu(3+) fluorescent band intensities is shown to be independent of Eu(3+) concentration and relatively free of interference. This robust method allows the presence of an interferent to be determined from the experimental data.
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