A generic FIA system for determination of enzyme activities : Assay of cellulase

The concept of a generic FIA system is described that allows multi-points assays of enzyme activities, that require a coupled chemical derivation reaction for detection. The enzymatic degradation procedure and the ensuing chemical indicator reaction are completely separated, which inherently allows each of these processes to be individually optimized with respect to operational parameters such as heating, cooling or possibly stopped flow manipulations. The applicability of the approach is demonstrated for the determination of the activity of cellulase using spectrophotometric detection via a couple chromogenic indicator reaction.