Related Experiment Video
Updated: Jun 28, 2026

Measuring Biomolecular DSC Profiles with Thermolabile Ligands to Rapidly Characterize Folding and Binding Interactions
Published on: November 21, 2017
Thermodynamic parameters for the molecular inclusion reaction of dulcin with beta-cyclodextrin. spectrofluorimetric
A N Díaz1, L S Feria, F G Sánchez
1Departamento de Química Analítica, Facultad de Ciencias, Universidad de Málaga, 29071-Spain.
Abstract:
The inclusion of dulcin in alpha- and beta-cyclodextrin has been studied by fluorescence spectroscopy. To quantitatively describe complex formation between the beta-cyclodextrin and dulcin, an association constant of 290 M(-1) at 21 degrees was obtained. The thermodynamics associated with the complex formation between dulcin and beta-cyclodextrin in aqueous solution has been studied. The obtained value of DeltaG(0) = -13.7 kJ/mole at 21 degrees , together with DeltaH(0) = -33.6 +/- 2.3 kJ/mole and DeltaS(0) = -67.2 +/-8.3 Jmole(-1) K(-1) indicate that dulcin has a very marked tendency to associate with beta-cyclodextrin in water. The inclusion complex of dulcin in beta-cyclodextrin has been used to determine dulein in the range 0.13-5 mug/ml the method has been applied to determine dulcin in soft drinks.
Related Concept Videos
Diels–Alder vs Retro-Diels–Alder Reaction: Thermodynamic Factors
Extraction: Partition and Distribution Coefficients
For extracting a solute from an aqueous phase into an organic...
UV–Vis Spectroscopy: Woodward–Fieser Rules
Cycloaddition Reactions: MO Requirements for Thermal Activation
Measuring Reaction Rates

