Gas-sensing internal enzyme fiber optic biosensor for hydrogen peroxide

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Enzyme Biosensors

Background:

• Hydrogen peroxide (H2O2) is a crucial biomarker in various biological processes and disease states.
• Accurate and selective detection of H2O2 is essential for clinical diagnostics and research.
• Existing H2O2 detection methods may face limitations in sensitivity, selectivity, or operational complexity.

Purpose of the Study:

• To demonstrate the feasibility of a novel gas-sensing, internal enzyme biosensing scheme for H2O2 measurement.
• To evaluate two horseradish peroxidase (HRP)-catalyzed reactions for H2O2 detection.
• To establish the selectivity and sensitivity of the proposed biosensing system.

Main Methods:

• A microporous Teflon membrane was utilized to separate the enzyme reaction from the sample solution.
• Two HRP-catalyzed reactions were employed, with product formation monitored by fluorescence and chemiluminescence.
• The rate of H2O2 diffusion across the membrane was correlated with sample H2O2 concentration.

Main Results:

• The biosensing scheme demonstrated feasibility for selective H2O2 measurement.
• Detection limits were estimated at 0.7 mM for fluorescence and 10 μM for chemiluminescence.
• The system showed selectivity for H2O2 over interfering substances like ascorbic acid, uric acid, and tyrosine.

Conclusions:

• The developed gas-sensing, internal enzyme biosensor provides a novel and effective method for H2O2 quantification.
• The HRP-based system exhibits good sensitivity and selectivity, making it suitable for H2O2 analysis.
• This approach holds potential for applications requiring precise and reliable hydrogen peroxide measurements.