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Updated: Jun 28, 2026

The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis
Published on: March 17, 2010
A PC compatible computer program for the calculation of equilibrium constants by the simultaneous processing of
V I Vetrogon1, N G Lukyanenko, M J Schwing-Weill
1A. V. Bogatsky Physico-Chemical Institute, Academy of Sciences of the Ukraine, 86, Chernomorskaya doroga, 270080, Odessa, Ukraine.
Abstract:
A new PC compatible computer program SIRKO for the calculation of the equilibrium constants by means of the simultaneous processing of different experimental data from various techniques is given. This program is characterized by the application of a universal response function, which allows many different physicochemical methods to be used to study equilibria without any modification of the program. It is also possible to refine additional parameters (electrode parameters, initial analytic concentration, etc). We have used the least-squares method with the weightings calculated according to the error propagation rule taking into account all independent variables. To minimize the function, singular Jacobian decomposition is applied. This allows control of its range and forms a set of parameters, determined from the experimental data.
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