Solid surface room temperature phosphorimetry analysis of reserpine in pharmaceutical formulations

Area of Science:

• Analytical Chemistry
• Pharmaceutical Analysis

Background:

• Reserpine analysis requires simple, rapid, and sensitive methods.
• Existing methods may lack efficiency or sensitivity for certain applications.

Purpose of the Study:

• To develop a novel analytical method for reserpine quantification.
• To establish a sensitive and rapid technique for pharmaceutical analysis.

Main Methods:

• Solid surface room temperature phosphorimetry was utilized.
• Reserpine hydrolysis in a basic medium induced phosphorescence emission.
• Chromatography paper served as the solid substrate, pre-treated for background reduction.
• Optimization involved testing heavy atom salts and sodium dodecyl sulfate for signal enhancement.

Main Results:

• A linear dynamic range spanning three orders of magnitude (10(-7)-10(-4)M) was achieved.
• A low limit of detection of 1.9 ng was estimated.
• High recovery rates (98.7% and 100.3%) were observed in two different pharmaceutical dosage forms.

Conclusions:

• The developed method is simple, rapid, and highly sensitive for reserpine analysis.
• The technique demonstrates excellent performance in quantifying reserpine within complex pharmaceutical matrices.
• Solid surface room temperature phosphorimetry offers a viable approach for quality control of reserpine-containing drugs.