Related Experiment Video
Updated: Jun 28, 2026
10:28
A Semi-High-Throughput Adaptation of the NADH-Coupled ATPase Assay for Screening Small Molecule Inhibitors
Published on: August 17, 2019
Determination of ATP using chelation-enhanced fluorescence
Talanta
|February 1, 1996
Summary
A novel fluorescence method accurately detects adenosine 5'-triphosphate (ATP) with minimal interference. This technique offers sensitive ATP quantification for various applications.
Area of Science:
- Analytical Chemistry
- Biochemistry
Background:
- Accurate adenosine 5 -triphosphate (ATP) quantification is crucial in biological and chemical analyses.
- Existing methods for ATP determination may suffer from limited sensitivity or significant interference.
Purpose of the Study:
- To develop a direct, sensitive, and selective method for ATP determination.
- To utilize chelation-enhanced fluorescence for improved analytical performance.
Main Methods:
- Developed a method employing the chelation-enhanced fluorescence between N-(anthracen-9 -yl methyl)tris(3-aminopropyl) amine and ATP.
- Evaluated the method in both batch and flow-injection analysis (FIA) configurations.
Main Results:
- The FIA mode achieved a typical detection limit of 1 $\mu$M for ATP.
- Demonstrated a linear dynamic range of 0.5–100 ppm for ATP.
- Reported a relative standard deviation of 2.3% at an ATP concentration of 20 ppm, indicating good precision.
Conclusions:
- The developed chelation-enhanced fluorescence method provides a sensitive and selective approach for direct ATP determination.
- The method is robust and suitable for application in both batch and flow-injection analysis systems.
- This technique offers a valuable tool for researchers requiring accurate ATP measurements with minimal interference.
