Polarographic determination of mitoxantrone in pharmaceutical preparations and biological media

An A.c. tast polarographic method for the determination of mitoxantrone in pharmaceutical preparations and biological media is described. The optimum pH for the extraction of the drug from various samples into a 4:1 mixture of chloroform-isopropanol is indicated. The effect of other parameters such as temperature, composition of supporting electrolyte and polarographic variables (drop time, a.c. voltage amplitude, voltage sweep rate) on the sensitivity and reproducibility of the method is also verified, and a simple and precise recommended method is proposed for the determination of mitoxantrone. The method is found to be applicable to the analysis of the drug in extracted phases at concentrations 5 x 10(-7) - 2.5 x 10(-5) M with a precision of about 1.5% (relative standard deviation). The limit of detection is calculated as 3.5 x 10(-7) M for both aqueous pharmaceutical preparations and biological media.