Adaptation of microthermal probes for the determination of biochemical species

A glucose sensitive enzymatic thermal sensor based on a pair of commercial microthermal probes was fabricated by cross-linking of the corresponding enzymes (glucose oxidase and catalase) with bovine serum albumin onto the chip surfaces. The characteristics of this sensor were optimised by testing it in different conditions. To find the maximal sensitivity, the calibration curves of this sensor were measured in different buffer solutions with and without the addition of H(2)O(2) to the analysed samples at various rates of sample flow stream. Different buffer solutions and hydrogen peroxide concentraions were used to realise the principles of a chemical and biochemical amplification of the biosensor response. It was shown that the level of the biosensor response and its dynamic range increase if hydrogen peroxide is added to the analysed samples and depend on the type of buffer solutions tested and the rate of the sample flow stream.