Cathodic stripping voltammetry of the anticancer agent 5-fluorouracil and determination in urine

Cathodic stripping voltammetry was used to determine 5-fluorouracil (5-FU) in the presence of traces of Cu(II). It was found that the addition of 5 x 10(-9) mol dm(-3) Cu(II) to the measurement cell greatly enhanced the peak current of the adsorbed molecule. Different parameters were tested to optimize the conditions for the determination of 5-FU. The adsorbed form is reduced irreversibly. It was observed that by controlling the deposition potential, the technique could be directed to the determination of Cu(II) or the drug. The linear range was from 5 x 10(-9) to 6 x 10(-8) mol dm(-3) for 5-FU and from 6 x 10(-9) to 5 x 10(-8) mol dm(-3) for Cu(II). Detection limits of 4.6 x 10(-10) and 5 x 10(-10) mol dm(-3) were obtained for 5-FU and Cu(II), respectively. The method was applied to urine and molecules or ions which may interfere were studied.