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Simultaneous voltammetric determination of uric and ascorbic acids in urine
E M Strochkova1, Y I Tur'yan, I Kuselman
1The National Physical Laboratory of Israel, Danciger A Building, Givat Ram, Jerusalem 91 904, Israel.
Talanta
|October 31, 2008
Summary
A new voltammetric method allows simultaneous detection of uric acid (UA) and ascorbic acid (AA) in urine. This method uses a glassy carbon electrode and offers satisfactory calibration, detection limits, and recoveries for both analytes.
Area of Science:
- Analytical Chemistry
- Electrochemistry
Background:
- Uric acid (UA) and ascorbic acid (AA) are important biomarkers in urine.
- Simultaneous determination of UA and AA presents analytical challenges due to their electrochemical properties.
Purpose of the Study:
- To develop and validate an anodic voltammetric method for the simultaneous determination of UA and AA in human urine.
- To optimize electrode pre-treatment and voltammetric techniques for enhanced sensitivity and accuracy.
Main Methods:
- Utilized a commercial rotating glassy carbon electrode for anodic voltammetry.
- Employed a buffer supporting electrolyte (HOAc+NH(4)OH; pH 5.1-5.2) for sample dilution.
- Optimized electrode pre-treatment (3 min at 0 V, 100 rpm) to adsorb inhibitors.
- Applied square-wave voltammetry for UA and differential pulse voltammetry for AA detection.
Main Results:
- Achieved satisfactory calibration curves, detection limits, and recovery rates for both UA and AA.
- Demonstrated successful simultaneous determination of UA and AA in diluted urine samples.
- Identified distinct optimal voltammetric techniques for each analyte (square-wave for UA, differential pulse for AA).
Conclusions:
- The developed voltammetric method provides a reliable approach for the simultaneous quantification of uric acid and ascorbic acid in urine.
- The method's efficiency in handling urine matrix inhibitors suggests its potential for clinical diagnostics.
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