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Updated: Jun 28, 2026
High-Throughput Measurement and Classification of Organic P in Environmental Samples
Published on: June 8, 2011
Spectrophotometric determination of isoproturon using p-aminoacetophenone and its application in environmental and
O Agrawal1, J V Das, V K Gupta
1School of Studies in Chemistry, Pt. Ravishankar Shukla University, Raipur 492010 (M.P.), India.
Abstract:
A simple and sensitive method for the determination of isoproturon, a widely used herbicide is described here which is based on alkaline hydrolysis of isoproturon to its corresponding primary amine followed by diazotization and coupling with p-aminoacetophenone in alkaline medium. The absorption maxima of the coloured compound formed is measured at 525 nm. Beer's law is obeyed over the concentration range of 0.5-5.0 mug in a final solution volume of 25 ml (0.02-0.2 ppm). The molar absorptivity and Sandell's sensitivity were found to be 8.6x10(5) l mol(-1) cm(-1) and 0.0002 mug cm(-2), respectively. The method has been satisfactorily applied to the determination of isoproturon in environmental and biological samples.
